Study on the viability of cooling canine semen using milk and egg yolk glycine extenders

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Isabel Candia Nunes da Cunha
Maria Denise Lopes

Abstract

Twenty-seven ejaculates of an adult, mixed breed, medium size dog were used in this study. The semen was collected by penile stimulation and divided into three equal samples. Group I (G I): cooled without extender; Group 2 (G2): diluted with skimmed milk-based extender; Group 3 (G3): diluted with glycine egg yolk-based extender, without glycerol. Samples were evaluated after collection (MO) as to sperm motility and vigor, and integrity of sperm membranes, and then incubated at 50 C. Samples were again evaluated for motility and vigor after being refrigerated for 24 (M 1),48 (M2) and 72 (M3) hours. M3 also included the integrity of sperm membranes in the evaluation. Statistical analysis of the obtained data showed G2 and G3 as equivalent, both being superior to G I in relation to the evaluated parameters. The cooling technique showed to be viable and the studied extenders were efficient in preserving the sperm functions during the cooling process of canine semen.

Article Details

How to Cite
DA CUNHA, I. C. N.; LOPES, M. D. Study on the viability of cooling canine semen using milk and egg yolk glycine extenders. Revista de Educação Continuada em Medicina Veterinária e Zootecnia, v. 3, n. 1, p. 37-42, 1 Jan. 2000.
Section
SMALL ANIMALS