Use of propidium iodide like a celular contrast staining in the direct fluorescent antibody (DFA) test for the rabies viruses diagnostic

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M. Iguala-Vidales

Abstract

Rabies remains one of the most important zoonosis worldwide and represents a serious problem in many countries. Into the diagnostic tools the direct fluorescent antibody (DFA) test is a fast and sensitive method to diagnosis rabies infection in animals and in humans (1, 3). The test is based on microscopic examination, under ultraviolet light, impressions, smear samples of tissue of the hippocampus (flagpole of Ammon), the cerebellum and the medulla or tissue sections; antibodies (IgG) used in the conjugate monoclonal allow specific and uniform coloring without interference from the fund. In cell biology, propidium iodide is used as a dye contrast that differentiates the nucleus from the cytoplasm (intercalates in DNA) (4), there is evidences of its use in stains for identification of Herpes Simplex Type 1 (5).

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How to Cite
IGUALA-VIDALES, M. Use of propidium iodide like a celular contrast staining in the direct fluorescent antibody (DFA) test for the rabies viruses diagnostic. Revista de Educação Continuada em Medicina Veterinária e Zootecnia, v. 11, n. 1, p. 51-51, 11.
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